Enzymes and Inhibitors
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 1600 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 100 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
New England Biolabs, Inc. Exonuclease T – 1250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
New England Biolabs, Inc. Enterokinase, light chain – 2560 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. beta-Agarase I – 100 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 1000 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. Factor Xa Protease – 50 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
New England Biolabs, Inc. RNase H – 250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
New England Biolabs, Inc. Pyrophosphatase, inorganic (yeast) – 50 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. A variety of metabolic reactions generate inorganic pyrophosphate as a reaction byproduct. Such reactions are rendered irreversible when the pyrophosphate is degraded by pyrophosphatase. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
Sigma Aldrich Fine Chemicals Biosciences Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Sigma Aldrich Fine Chemicals Biosciences Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN
Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN